Enzymes — Temperature & pH
Enzyme activity56%
Temperature effect56%
pH effect100%
StateIntact
- Get the enzyme working — above 50% activity
- Cool it down — make it sluggish (below 40%) with a low temperature
- Find the optimum — get activity above 95%
- Denature it with heat — drive activity below 5% using a temperature above 48 °C
- Prove pH matters: with the temperature at the optimum, use pH alone to drop activity below 15%